Multisectoriel Resistance aux antimicrobiens multi-secteurs

Genetic Diversity, Extended-Spectrum Beta-Lactamase (ESBL) Screening, and Potential Public Health Implications of Gram-Negative Bacteria Recovered from Man-Made Lakes and Surrounding Vegetables

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Dr FOSSOU Kouakou Romain
INP-HB — Institut National Polytechnique Félix Houphouët-Boigny
· Ahou Cinthia Inès Yebouet 1, Kouakou Romain Fossou 1 , Zaka Ghislaine Claude Kouadjo-Zézé 2 Anthony Ifeanyi Okoh 3,* andAdolpheZézé1

Résumé

The emergence and dissemination of extended-spectrum beta-lactamase (ESBL)-producing bacteria pose a major public health threat, necessitating a One Health approach to addressing this threat. Thus, the diversity, ESBL production, and potential public health implications of Gram-negative bacteria recovered from man-made lakes and surrounding lettuce in Yamoussoukro, Côte d’Ivoire were assessed in this study. Also, the lakes’ physicochemical parameters were assessed and correlated with bacteria community using Pearson correlation. A total of 68 Gram-negative bacterial isolates were recovered from the samples and identified via 16S rDNA gene sequencing. Phylogenetic analysis suggested multiple genus-/species-level variations within the isolates. Escherichia coli was the most prevalent in lake water (39.5%), while Acinetobacter was the dominant genus in lettuce (30%). E. coli isolates showed high resistance to ampicillin (90.9%), cefepime (72.7%), cefotaxime (68.2%), and aztreonam (63.6%). Moreover, ESBL production was confirmed in E. coli isolates (22.05%), predominantly mediated by the blaCTX-M gene. Multidrug-resistant phenotypes were widespread, yielding similar multiple antibiotic resistance index (MARI) values in water (0.27–0.63) and lettuce (0.27–0.81). These data indicate high environmental contamination, which unfortunately is not being taken into account by lettuce producers according to an interview. Statistical analyses showed a significant relationship between bacterial diversity and lakes’ physicochemical parameters, including dissolved oxygen, pH, and turbidity. The basic education level of farmers, the prevalence of ESBL-producing E. coli, and the high prevalence of MDR Gram-negative bacteria in both environmental and crop sources in Yamoussoukro underscore the need for both integrated surveillance and management strategies to mitigate potential microbial public health risks within a One Health framework.

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Multisectoriel Systemes alimentaires durables

EVALUATION OF PRACTICES AT RISK OF MICROBIAL CONTAMINATION IN THE PRODUCTION CHAIN OF VEGETABLE SALADS SOLD IN PUBLIC CATERING IN THE SUBURBAN AREA OF ABIDJAN, IVORY COAST

In Côte d'Ivoire, the absence of good hygiene practices in certain sectors is common, especially in the market gardening sector, which could increase the risk of collective food poisoning. The objective of the study was to evaluate the practices and risks of microbial contamination, in the production chain of the suburban area of Abidjan. A survey was carried out using a questionnaire with 575 people involved in the sector, in particular producers, sellers, restaurateurs and consumers on the Anyama, Adiopodoumé, Bonoua and Dabou sites. On the various sites of the study, 70% of market gardening is practiced by men, 80% of whom do not have a level of education for compliance with hygiene rules. The producers use 95% of the untreated water emanating from wells, ponds and lagoons to water the vegetables. The sites lack sanitary facilities (80%), so the producers go to the saddle near the fields. In the markets, saleswomen relieve themselves in public toilets (84.4%) by washing their hands with soap-free water (64.1%). Restaurateurs serve pre-cut vegetables (52%) and stored at room temperature for three to four hours (67.2%) before consumption. Consumers claim to consume vegetable salads (61.7%) referably at breakfast (57%) and at least once a day (66%). Constant monitoring of the application of hygiene rules must be established from the producer passing through the markets to the consumer's plate, in order to prevent the appearance of food poisoning of vegetables and guarantee the health of all.

K
Dr KONAN Kouadio Fernique
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